Magnetic circular dichroism studies. XXV. A preliminary investigation of microsomal cytochromes.

نویسندگان

  • P M Dolinger
  • M Kielczewski
  • J R Trudell
  • G Barth
  • R E Linder
  • E Bunnenberg
  • C Djerassi
چکیده

The application of magnetic circular dichroism as an optical probe for simultaneous identification and determination of at least two microsomal cytochromes is demonstrated. The assignments of the bands in the spectra of microsomal suspensions are made from the spectra of soluble preparations of cytochrome P-450 obtained from Pseudomonas putida and of cytochrome b(5) obtained from rat livers.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Magnetic circular dichroism studies XXXV. A comparison of cytochromes P-450 and P-448.

Cytochromes P-450 and P-448 in microsomal suspensions have been shown to be spectrally distinct by magnetic circular dichroism spectroscopy. Furthermore, this technique can be used to measure induction of these two cytochromes by phenobarbital and 3-methyl-cholanthrene. Magnetic circular dichroism spectroscopy is thus at least as useful as difference spectroscopy for the investigation of P-450 ...

متن کامل

In vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy

In the near future, design of a new generation of drugs targeting proteins will be required. Considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. So far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. In these studies, use of spectroscopic method...

متن کامل

Formation of high spin cytochrome a in isolated cytochrome c oxidase.

The effect of temperature on the electronic structure of cytochromes a and a3 has been determined at two different concentrations of the solubilizing detergent, N-dodecyl beta-D-maltoside. In each case, an incubation temperature of 40 degrees C resulted in a 20-30% increase in the 430-nm conformer of cytochrome a3 when compared with a 20 degrees C control, but the higher detergent concentration...

متن کامل

First principles investigation of the validity and range of applicability of the x-ray magnetic circular dichroism sum rule.

First Principles Investigation of the Validity and Range of Applicability of the X-Ray Magnetic Circular Dichroism Sum Rule Ruqian Wu, ~ Dingsheng Wang, and A. J. Freeman~ 'Department of Physics and Astronomy, Northtvestern University, Evanston, Illinois 60808 Institute of Physics, Academia Sinica, Beijing 100080, People's Republic of China Materials Science Division, Argonne National Laborator...

متن کامل

Magnetic circular dichroism of ferrous carbonyl adducts of cytochromes P-450 and P-420 and their synthetic models: further evidence for mercaptide as the fifth ligand to iron.

Absorption and magnetic circular dichroism (MCD) spectra have been obtained for the ferrous carbonyl adducts of cytochromes P-450 and P-420 as well as synthetic model systems. Ferrous porphyrins with sodium methyl mercaptide and CO in benzene give MCD and absorption spectra which are almost identical to those of the natural enzyme, indicating that in P-450 a mercaptide serves as the fifth ligan...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 71 2  شماره 

صفحات  -

تاریخ انتشار 1974